Biochemistry

Overview

GOAL
The broad goal of the teaching of undergraduate students in biochemistry is to make them understand the scientific basis of the life processes at the molecular level and to orient them towards the application of the knowledge acquired in solving clinical problems.

OBJECTIVES
- KNOWLEDGE
  At the end of the course, the student should be able to :
  1. Describe the molecular and functional organization of a cell and list its subcellular components;
  2. Delineate structure, function and inter-relationships of biomolecules and consequences of deviation from normal;
  3. Summarize the fundamental aspects of enzymology and clinical application wherein regulation of enzymatic activity is altered;
  4. Describe digestion and assimilation of nutrients and consequences of malnutrition;
  5. Integrate the various aspects of metabolism and their regulatory pathways;
  6. Explain the biochemical basis of inherited disorders with their associated sequelae;
  7. Describe mechanisms involved in maintenance of body fluid and pH homeostasis;
  8. Outline the molecular mechanisms of gene expression and regulation, the principles of genetic engineering and their application in medicine;
  9. Summarize the molecular concepts of body defence and their application in medicine;
  10. Outline the biochemical basis of environmental health hazards, biochemical basis of cancer and carcinogenesis;
  11. Familiarize with the principles of various conventional and specialized laboratory investigations and instrumentation analysis and interpretation of a given data;
  12. The ability to suggest experiments to support theoretical concepts and clinical diagnosis.
- SKILLS
  At the end of the course, the student should be able to:
  1. Make use of conventional techniques/instruments to perform biochemical analysis relevant to clinical screening and diagnosis;
  2. Analyze and interpret investigative data;
  3. Demonstrate the skills of solving scientific and clinical problems and decision making;
- INTEGRATION
  The knowledge acquired in biochemistry should help the students to integrate molecular events with structure and function of the human body in health and disease.

Facilities

1) Under Graduate Biochemistry Laboratory

LIST OF INSTRUMENTS IN Undergraduate Biochemistry LABORATORY

Sr.No.	Item
1	Fume cupboards
2	Boiling water bath with lids having 12 holes
3	Autoclave electric
4	Balance open pan
5	Digital Analytical Balance
6	Vortex mixer
7	Urinometer
8	Constant temp. water bath 6 holes/12 holes
9	Hot air oven
10	Pump vacuum
11	Colorimeters
12	Thermometers 0-250 °C
13	Thermometers 0-110 °C
14	Stop watch
15	Water distillation plant(metallic)
16	Centrifuge clinical for 12 tubes
17	Homogenizer
18	Microscope
16	Tools for small workshop for glass blowing and mending
17	Bottle dispenser
18	Vacutainer tube
19	Samplers different volume range
20	Glass ware as required by standard lab
21	Semi-automated analyzer

2) Clinical Biochemistry Laboratory

A) LISTOF EQUIPMENTS IN CLINICAL BIOCHEMISTRY LABORATORY

Sr. No.	NAME OF EQUIPMENT
1	Microlab,Semi Automated Biochemistry Analyzer
2	Hotair Oven
3	Incubator
4	Centrifuge Machine
5	Mispa i3 Nephelometer
6	Abbott Architect c4000 Fully automated analyzer
7	Abbott Architect i1000 Fully automated immunoassay analyzer

B) Scopsavailable at facility

SR NO.	PARAMETER (TESTS)	SR NO.	PARAMETER (TESTS)
1	GLUCOSE	25	TOTAL CHOLESTEROL
2	ALP	26	TRIGLYCERIDE
3	ALT	27	HDL-C
4	AST	28	ldl cHOLESTEROL
5	BILI TOTAL	29	vldl cHOLESTEROL
6	BILI Direct	30	S. Phosphorus
7	BILI Indirect	31	S. Magnesium
8	TOTAL PROTEIN	32	Urine albumin
9	ALBUMIN	33	hs TROPONIN-I
10	Globulin	34	S. TOTAL T3
11	CK TOTAL	35	S. TOTAL T4
12	CK-MB	36	S. TSH
13	UREA	37	TOTAL psa
14	CREATININE	38	Vitamin B12
15	FE-PI (IRON)	39	Vitamin D3
16	TIBC (UIBC)	40